Lactophenol cotton blue stain fungal element stain. Use of lactophenol cotton blue mounts of corneal scrapings as an. Organisms suspended in the stain are killed due to the presence of phenol. What safe alternatives are there to lactophenol cotton blue for fungal. A small part of fungal colony was taken from potato dextrose agar pda plate using a sterile needle and it was placed on the drop of lactophenol cotton blue that had been taken on the glass slide.
Hardy diagnostics lactophenol cotton blue stain is recommended for mounting. Identification of the substancemixture and of the companyundertaking 1. What is the role of phenol in lactophenol cotton blue. Ten milliliters of a 7% solution of phenol contains 0. Cotton blue is an acid dye that stains the chitin present. Hermetically sealed in ampule oneday supply dropbydrop dispensing directions to perform tests, squeeze the dropper to crush the ampule. Bluemount, permanent fungal stain, lactophenol cotton blue with pva, 15ml each, by hardy diagnostics, prefer to ship ground.
Cotton blue lactophenol page 1 of 8 material safety data sheet according to 91155ec section 1. Composition, preparation and uses september 10, 2016 3. State the scientific name of the yeast studied in the laboratory you will use yeast over and over in. Lactophenol cotton blue used for wet mounts in the examination of yeasts and molds, serving as mounting fluid and stain. Summary and explanation linder recommended lactophenol as a mounting fluid for examination and preservation of fungi. Mix the specimen whether a skin scraping, fluid exudate or tissue with two drops of the 10% koh on a clean slide. Comparison of microscopic morphology of fungi using. Jul 20, 2014 in this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method for identification of filamentous fungi. Lactophenol blue solution for microscopy, for staining molds. This item is sold and shipped to schools and businesses only. Page 17 safety data sheet according to whs regulations printing date 29.
Methyl blue is also available in mixture with water blue, under name aniline blue ws, aniline blue, china blue, or soluble blue. Molds examined in teased mounts must be well teased in order. Relevant identified uses of the substance or mixture and uses advised against identified uses laboratory reagent. Lactophenol cotton blue solution mdl no mfcd00146574 density. Lactophenol anilin blue solution, lactophenol cotton blue solution 635. Lactophenolcotton blue solution, readily available from biological supply houses, stain fungi blue only living tissue picks it up. Jan 11, 2020 the lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. We generally do not inject more than 15 ml of 7% phenol in a session. Lpcb100, lpcb500, lpcba100, lpcba500 recommended use of the chemical and restriction on use. Slide culture technique and lactophenol cotton blue staining.
New staining technique for fungalinfected plant tissues. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of. Remel bactidrop lactophenol aniline blue is for staining fungal elements. Jorvet j326b lactophenol cotton blue stain for animal use. Properties, disease and laboratory diagnosis april 27, 20 1. Lactophenol cotton blue stain fungal element stain for mycology. The cotton blue is a histological stain which turns the fungal cell walls blue. Pdf evaluation of lactophenol cotton blue for wet mount. Staining in 5% blue cotton in lactophenol for 20 min macedo, 1997. What safe alternatives are there to lactophenol cotton. Product should not be used if there are any signs of contamination. Safety data sheet bactidroptm lactophenol aniline blue. Lactophenol blue solution lactophenol cotton blue solution.
Our lactophenol blue stain is used as a mounting medium. Lactophenol cotton blue lpcb mounts of corneal ulcer scrap ings were. Bd diagnostic 261188 dropper lactophenol stain, cotton blue pack of 50. Material safety data sheet section 1 product and company information product name. Lactophenol picric acid s017 intended use lactophenol picric acid is used as fungal staining solution. Add a drop of lactophenol cotton blue solution on a slide. Lactophenol aniline blue intended use remel lactophenol aniline blue is a stain recommended for use in wet mount preparations for microscopic examination of fungi. Lactophenol, staining solutions, by loba chemie, india. Lactophenol cotton blue is used for staining solution for fungi. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material, e. A small part of fungal colony was taken from potato dextrose agar pda plate using a sterile needle and it was placed on the drop of lactophenol cotton blue that had been taken on. Lactophenol blue solution is a mounting medium and staining agent used in the.
Preparation of lactophenol cotton blue slide mounts. Search results for lactophenol cotton blue at sigmaaldrich. The lpcb also serves as a mounting medium for placing on a cover slip. This products resides on a fisher scientific gsa or va contract. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Dropper contains oneday supply of reagent or stain, hermetically sealed inside an ampule. Uses advised against no specific uses advised against are identified.
Lactophenol cotton blue stain fungal element stain for. Some article used methylen blue stain but i dont know about the concentration that i should use. Central drug house p ltd 728 vardaan house new delhi2 india. To perform tests, squeeze the dropper to crush the ampule. Procedure a drop of lactophenol cotton blue was placed on a clean glass slide. The lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. Bbl lactophenol cotton blue stain droppers are used in wet amounts for examination of fungi 50sp, 500ca. Slide culture technique and lactophenol cotton blue. Pdf the use of lactophenol cotton blue lpcb stain for wet mount.
Staining techniques in microbiology linkedin slideshare. On the first day, dissolve the cotton blue in the distilled water. A solution of phenol and lactic acid in glycerol and water. Z7 the hardy diagnostics manufacturing facility and quality management system comply with the fdas good manufacturing practices gmp and have achieved iso 485 certification as a medical device.
Lactophenol helps preserve the fungal structures and kill any microorganisms. Diagnosis of dermatophytoses is based on the characteristic gross appearance of the lesions, cytologic examination of hairs or skin scrapings treated with potassium hydroxide examined with contrast microscopy or stained with lactophenol cotton blue, histologic examination of skin biopsy table 2. In this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method. Stain fungal elements with thermo scientific remel lactophenol aniline blue. Examine under microscope using 10x and 40x objective. Lactophenol cotton blue is used as staining solution for fungi.
Supplied in crushable ampules enclosed in a protective plastic sheath with a convenient dropper dispenser tip. I read some of journal and they used lactophenol cotton blue stain but its hard to find it here. Dermatophyte test medium an overview sciencedirect topics. Lactophenol cotton blue lcb is a mixture of methyl blue, a histological stain, and lactophenol it is used in wetmount preparations for visualization of fungal. The fungi identified by characteristic microscopic morphology such as shape, size, arrangements of spores and hyphae. The material is stained in one step with lactophenol blue solution. Composition ingredients formula adjusted, standardized to suit performance parameters directions principle and interpretation fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. State the scientific name of the yeast studied in the laboratory you will use yeast over and over in this lab so know this cold for exams and quizzes. Stain airdried, heatfixed smears of bacteriological material, e.
Lactophenol cotton blue solution, readily available from biological supply houses, stain fungi blue only living tissue picks it up. Lpcb is a stain used for making semipermanent microscopic preparation of fungi. The lethal range of phenol that gets into the central nervous system is approximately 8. For pigmented strains of moulds, lactophenol without cotton blue is available and can be used quality control internal quality control of the lactophenol blue solution must be performed regularly on known reference organisms to ensure the performance of the mounting solution. Jorvet j326b lactophenol cotton blue stain indications.
Dissolve the cotton blue in distilled water and leave overnight to eliminate insoluble dye. Lactophenol cotton blue stain other means of identification. Lactophenol cotton blue wet mount is simple and widelthe y used staining method for fungi. Other information msds first issued 28022007 msds data revised 27092007 uses stain for fungal elements. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Lactophenol blue solution, for microscopy, for staining molds milliporesigma synonyms. Add one or two drops of lactophenol cotton blue stain before alcohol gets off. Lactophenol blue solution is readytouse staining solution for fungi staining in bacteriology with samples of human origin.
What safe alternatives are there to lactophenol cotton blue. The yeast with lactophenol cotton blue is called a simple stain because it stains the yeast cells, so they can be more readily observed under the microscope. Milliporesigma lactophenol blue solution lactophenol blue. Lactophenol cotton blue lpcb wet mount preparation it is used to demonstrate fungi and fungal elements under the microscope in lpcb wet mount preparation. Lactophenol blue solution stain fungal, sigmaaldrich vwr. Immediately flush eyes with gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids occasionally. A drop of lactophenol solution is placed onto a clean glass microscope slide. Place a drop of seventy percent alcohol on a clean microscope slide. Proslide microscope slides, frosted, premium, 3x1 inches x 1mm, 72 per box, by hardy diagnostics. Invert the dropper for convenient dropbydrop dispensing. Prepare lactophenol cotton blue wet mount of fungal colony. Place suspected fungi on a glass slide, drop lactophenolcotton blue solution on it, cover it with a cover slip and examine with an optical microscope. Place the coverslip on the drop avoiding air bubbles to be trapped. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi.
Bd diagnostic 261188 dropper lactophenol stain, cotton. Lactophenol cotton blue solution material safety data. Lactophenol cotton blue stain definition of lactophenol. Oct 18, 2016 procedure a drop of lactophenol cotton blue was placed on a clean glass slide. Product name lactophenol cotton blue product number pl. Manual of clinical microbiology, american society for microbiology, washington, d. Lactophenol aniline blue may be adequate for presumptive identification, however, colonial morphology and further microscopic examination may be necessary for definitive identification. Lactophenol cotton blue lpcb for the staining and microscopic identification of fungi. Lactophenol cotton blue, fungal stain, 15ml, by hardy diagnostics. If you are viewing this page as a nonregistered user, the prices displayed is list price. Consult appropriate references for further instructions. Bactidroptm lactophenol aniline blue safety data sheet date of issue 27092007. The lactophenol cotton blue lpcb wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. Wet mount preparation is the most widely used method of staining and observing fungi.
If a phenol block seems ineffective after several weeks, it may be repeated. Sigmaaldrich 3050 spruce street saint louis mo 63103 usa telephone. Lactophenol anilin blue solution, lactophenol cotton blue solution. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material. Product name lactophenol blue solution sigmaaldrich. Check for and remove contact lenses if present and easy to do. Manual of clinical mycology prepared under the auspices of the division of medical. The slides were analysed, and the images were captured with a leica dc 300f digital camera attached to a leica dmld microscope. Preparation of lactophenol cotton blue lpcb slide mounts. Bd diagnostic 261188 dropper lactophenol stain, cotton blue.
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